Determination of clocapramine and its metabolites in plasma by automated column-switching high performance liquid chromatography

Kozo HIKIDA, Yoshimasa INOUE, Norio KOJIMA, Yosuke OHKURA
1990 Analytical Sciences  
An automated column-switching high performance liquid chromatographic method with ultraviolet detection is described for rapid and sensitive determination of clocapramine, a psychotropic drug, and its two metabolites, clospipramine and dehydroclospipramine, in dog and human plasmas. The drug and the metabolites were extracted together with trifluperidol as an internal standard with a heptane-chloroform mixture from plasma. The extract was subjected to column-switching high performance liquid
more » ... rformance liquid chromatography using a bovine serum albumin-coated cyanopropyl silica gel column, a newly developed sample clean-up column, and a reversed phase column (TSK gel ODS-80TM) for analytical separation. The detection limits (S/N=3) of clocapramine, clospipramine and dehydroclospipramine were 0.5 -1.0 ng ml-1. Keywords Column-switching high performance liquid chromatography, clocapramine, metabolite, dehydroclospipramine, plasma, bovine serum albumin-coated cyanopropyl silica gel column clospipramine,
doi:10.2116/analsci.6.367 fatcat:nh27haxt4jhkjetg65tof6a4kq