INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY Fermenting Cell Walls of Processed Sugarcane Pith by Ruminal Bacteria, Protozoa and Fungi
The objectives of this study were to determine contribution of ruminal bacteria, protozoa and fungi to the fermentation of cell walls of sugarcane pith treated with low temperature steam (121ºC, 120 min.) and or sulfuric acid (0.0, 6, 12 & 18 g/kg DM), and effectiveness of this method for increasing the feed value of sugarcane pith and negative effects of this method on rumen microorganisms. Gas production parameters were determined and cumulative gas production data were fitted to the
... ted to the exponential equation. The results showed that processing with low temperature steam resulted in significant increase in rate (C) and potential gas production (B) by all microbial fractions, as the highest (B) (112.9 mL) was for condition 18 g/kg of dry matter (DM) acid. The highest gas produced by total microorganism and rumen isolated bacteria was followed by fungi and protozoa fractions. In vitro cell wall degradation, organic matter digestibility (OMD) and metabolisable energy (ME) of sugarcane pith treated with steam and 18 g/kg acid was the highest and microbial biomass production (MB) was the lowest for all microbial groups. Therefore, it appears that the gas production parameters and degradability of sugarcane pith by rumen microbial fractions are influenced by low temperature steam and sulfuric acid treatment, bacterial group generates a larger volume of gas production than fungal or protozoan group and this method had no negative effect on rumen microorganisms.