The effects of low level laser therapy on injured skeletal muscle

Ana Claudia Muniz Renno, Lívia Assis, Beatriz Peres, Natalia Camargo Rodrigues, Roberta de Matos Brunelli, Renata Luri Toma, Carla Christina Medalha, Daniel Araki Ribeiro
2014 Brazilian Archives of Biology and Technology  
The main purpose of the present study was to investigate the effects of low-level laser therapy (LLLT) used in two different fluencies on injured skeletal muscle after cryolesion by means of histopathological analysis and immunohistochemistry for COX-2. A total of sixty male Wistar rats were randomly distributed into three groups: injured animals without any treatment; 808 nm laser treated group, at 10 J/cm² and 808 nm laser treated group, at 50 J/cm². Each group was divided into two different
more » ... into two different subgroups (n=10) on days 6 and 13 post-injury. The results showed that the animals irradiated with laser at 10 J/cm² or 50 J/cm² presented the areas with cell infiltrate and pointed out to minor and mild areas with destroyed zones compared with the control group. Also, a COX-2 downregulation was noticed in the groups exposed to laser at two fluences evaluated in this study. Significant statistically differences (p<0.05) were noticed to collagen deposition in the laser treated animals, with the fluence of 50 J/cm 2 when compared to the other groups on day 13 post-surgery. Taken together, these results suggested that laser therapy could have positive effects on muscle repair in the rats after cryolesion.
doi:10.1590/s1516-89132014000100008 fatcat:bno5yutuobbltgpgtbysw36ugy