PARASITOLOGICAL SURVEY AND ANTIMICROBIAL SUSCEPTIBILITY PATTERNS OF MICROBIAL ISOLATES FROM PRE WASHED FRUITS AND VEGETABLES COLLECTED IN METTU TOWN, I/A/BORA ZONE, SOUTHWEST ETHIOPIA

Eshetu Chilo*, Tsegaye Berkessa
2018 Zenodo  
Intestinal parasitic infections are widely distributed throughout the world causing substantial intimidation to the public health, economy, and physical and cognitive development particularly among children in developing countries like Ethiopia. Fruits and Vegetables may be contaminated at any point during growing, harvesting, sorting, packaging, and storage. This study was aimed at assessing parasitological survey and antimicrobial susceptibility patterns of microbial isolates from fruits and
more » ... es from fruits and vegetables collected in Mettu town, Ethiopia from March –October, 2018.Methods:A cross sectional study design was conducted. A total of 240 fresh fruits and vegetable samples were collected on different days from local markets. Four types of fresh vegetable comprising lettuce, carrot, tomato, green pepper and two type fruits comprising lemon and banana were collected from selected local markets. For microbiological analyses, 25g of sample was aseptically weighed and washed gently in 225ml of sterile 0.1% (w/v) peptone water (Oxoid) for 3 minutes. Bacterial isolation and antimicrobial susceptibility was performed. For parasitological survey a portion fruit and vegetable was washed separately in 500 mL of normal saline for detaching the parasitic stages (ova, larvae, cysts) of helminthes and protozoan parasites commonly assumed to be associated with vegetable contamination. After overnight sedimentation of the washing solution, 15 mL of the sediment was then transferred to a centrifuge tube using sieve, to remove undesirable matters. For concentrating the parasitic stages, the tube was centrifuged at 3000 rpm for five minutes. After centrifugation, the supernatant was decanted carefully without shaking. Then the sediment was agitated gently by hand for redistributing the parasitic stages. Finally, the sediment was examined under a light microscope using ×10 and ×40 objectives. Results: A t [...]
doi:10.5281/zenodo.1495145 fatcat:clmety6mzzhlvewcw6v36b3vpa