Comparison of DNA Adducts from Exposure to Complex Mixtures in Various Human Tissues and Experimental Systems

Joellen Lewtas, Judy Mumford, Richard B. Everson, Barbara Hulka, Tim Wilcosky, Walter Kozumbo, Claudia Thompson, Michael George, Lubomir Dobias, Radim Sram, Xueming Li, Jane Gallagher
1993 Environmental Health Perspectives  
DNA adducts derived from complex mixtures of polycyclic aromatic compounds emitted from tobacco smoke are compared to industrial pollution sources (e.g., coke ovens and aluminum smelters), smoky coal burning, and urban air pollution. Exposures to coke oven emissionsand smoky coal, both potent rodent skin tumor initiators and lung carcinogens in humans, result in high levels of DNA adducts compared to tobacco smoke in the in Wtm calf thymus DNA model system, in cultured lymphocytes, and in the
more » ... cytes, and in the mouse skin assay. Using tobacco smoke as a model in human studies, we have compared relative DNA adduct levels detected in bloodlymphocytes, placental tissue, bronchoalveolar lung lavage cells, sperm, and autopsy tissues ofsmokers and nonsmokes Adduct levels in DNA isolted from smokers were highest in human heart and lung tissue with smaller but detectable differences in placental tissue and lung lavage cells. Comparison ofthe DNA adduct levelsresultngfrom human exposuretodifferentcompke mixturesswsthatemis fhomcokeovens, aluminum smelters, and smoky coal result in higher DNA adduct levels than tobacco smoke exposure. These studies suggest that humans exposed to complex combustion mixtures will have higher DNA adduct levels in target cells (e.g., lung) as compared to nontarget cells (e.g., lymphocytes) and that the adduct levels will be dependent on the genotoxic and DNA adductforming potency of the mixture.
doi:10.2307/3431463 fatcat:53qkn5os3rgkhjqf6cixyjwx4m