Fluorescence endomicroscopy with structured illumination

Nenad Bozinovic, Cathie Ventalon, Tim Ford, Jerome Mertz
2008 Optics Express  
We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (∼ 2.6μm) optically sectioned fluorescence images over a field of view of about 240μm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifacts that arise when imaging in thick tissue that exhibits significant out-of-focus background. To establish the potential of
more » ... the potential of our endomicroscope for preclinical or clinical applications, we provide images of BCECF-AM labeled rat colonic mucosa.
doi:10.1364/oe.16.008016 pmid:18545511 fatcat:7bdah5df2vfythphpkutdjlpbe