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Plasmid DNA is being used successfully as a gene delivery vector in a variety of clinical applications. Similar to other pharmaceutical products for clinical use, the plasmid vectors must meet rigorous purity standards. One important contaminant is the DNA of the host cell used to produce the plasmids. We have developed a new method to accurately quantitate E. colihost-cell DNA in plasmid preparations. This method is based on kinetic PCR using the ABI P RISM ® 7700 with 23S rDNA as a target.doi:10.2144/99263rr03 pmid:10090994 fatcat:wuv2caxmp5agxpyibycqqpripe