REGULATION OF ADIPOGENESIS BY MANGOSTEEN PEEL EXTRACT AND XANTHONES IN 3T3-L1 CELLS

2019 Biotropia: The Southeast Asian Journal of Tropical Biology  
33 Obesity is one of the risk factors for atherosclerosis. Fat accumulation and adipocyte 34 differentiation are associated with the occurrence and development of obesity. Thus, 35 suppression of adipocyte differentiation provides a potential anti-obesity approach. This study 36 examined the effect of mangosteen pericarp extract (MPE) and xanthone (α-Mangostin (AM) 37 and γ-Mangostin (GM)) on the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and 38 FAS in 3T3-L1 cells. Concentrations of
more » ... Concentrations of MPE and xanthones used were based on cytotoxic 39 assay on 3T3-L1 cells. Three different MPE concentrations (0, 25, and 50 µg/ml) were used in 40 this study. Likewise, three different concentrations of AM (0, 25, and 50 µM) and GM (0, 50, 41 and 75 µM) were also used in the experiment. The expressions of PPARγ, C/EBPα, SCD1, 42 LPL, aP2, adipoQ, and FAS genes were measured using real-time quantitative PCR. The 43 expression of the genes was down-regulated in the group of cells treated with 50 µg/ml of MPE 44 and 50 µM of GM. However, 25 µM and 50 µM of AM did not suppress PPARγ and SCD-1 45 expression. 50 µM of AM also failed to reduce aP2 gene expression. In conclusion, MPE and 46 GM showed potential anti-adipogenesis and anti-obesity effects by suppressing the expression 47 of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS genes in 3T3-L1 cells. 48 49
doi:10.11598/btb.2020.27.1.932 fatcat:pjplr26z7fgdhg2tnptuiyxdne