OPTIMIZATION AND BIOCHEMICAL CHARACTERIZATION OF THERMO-ALKALOTOLERANT CELLULASE ENZYME FROM ASPERGILLUS TERREUS PPCF

HEENA PARVEEN, NEHA BISHT, LAKSHMI TEWARI
2020 Cellulose Chemistry and Technology  
The aim of this study was to boost the production rate of a novel thermo-alkalotolerant cellulase (FPase) enzyme from the fungal isolate Aspergillus terreus PPCF. Initially, the extracellular FPase activity was 0.166 ± 0.03 UmL-1 in the culture filtrate, which further increased up to 0.91 ± 0.13 UmL-1 under optimized conditions (3% w/v wheat bran and 0.5% w/v ammonium sulfate). Through response surface methodology, the FPase activity was enhanced to 10.78 UmL1 under the cumulative effect of
more » ... ative effect of different factors. The zymogram analysis revealed only one activity band, with the molecular weight of ~110 KDa. The optimum pH was 7.0, but the enzyme was stable in a pH range of 4-10. The optimum temperature was 50 °C, but the stability range of the enzyme was 30-90 °C. The maximum effect of Mg2+ was observed on the FPase enzyme. The findings of the present study demonstrate the thermo- and alkalotolerance of the obtained cellulase enzyme, which will be beneficial to the biofuel and other industries.
doi:10.35812/cellulosechemtechnol.2020.54.93 fatcat:qognjumvofhvbgdv3kewu3upqe