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Milk samples from 237 Holstein-Friesian (HF) and 177 Simmentaler cows in six herds were typed for genetic variants of α S1 -, βand κ-caseins (Cn) and β-lactoglobulin (-Lg) using ultrathin-layer polyacrylamide isoelectric focusing in the presence of carrier ampholytes (3-10 pH). The frequency distribution of genetic variants of all four milk protein systems was different in HF and Simmentaler. In addition, milk production traits of the dairy cows were compared according to the genetic variantsdoi:10.4314/sajas.v35i1.4047 fatcat:xhz7ixw6pbelfa4n5zspjvhiuy