METHODOLOGICAL ASPECTS OF ATTEMPTS TO TRANS-DIFFERENTIATE ADULT STEM CELLS INTO EMBRYONIC-LIKE CELLS IN VITRO

Petr Uher, Petra Baborova, Renata Huttelova, Milena Kralickova, Pierre Vanderzwalmen, Nicolas Zech
2008 Biomedical Papers of the Faculty of Medicine of Palacky University  
Aims: The aim of this research was to set up an in vitro system to trans-diff erentiate haematopoietic stem cells (HSCs) into embryo-like stem cells in order to de-diff erentiate them. In this more naive state they should be cultivated more easily in order to augment them for consecutive diff erentiation and autologous transplantation for use in clinical practice. Methods: Using the principle of the methodology of blastocyst injection, HSCs were co-cultivated with mouse embryonic stem cells
more » ... onic stem cells (mES) with and without cell to cell contact. After co-cultivation HSCs were analyzed by fl owcytometry using haematopoietic markers (CD34, CD45, CD133) and embryonic stem cell markers (SSEA-4, Tra-1-60, Tra-1-81). Results: No ES cell markers were detected on the former HSCs. A decrease in HSC marker intensity was the only fi nding. This implies that no de-diff erentiation took place. Conclusions: We hypothesize that the unnatural situation of a mixture of two cell types originating in diff erent species may have led to this outcome. To achieve our goal of in vitro de-diff erentiation we need to use a purely human culture system without animal additives.
doi:10.5507/bp.2008.035 pmid:19219212 fatcat:wvxnczii6fc2fbv3f4vbvoaw3q