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Enzymatic assay, based on oxidation-reduction reaction catalyzed by alcohol dehydrogenase, is one of the methods used to determine ethanol concentration. The present study was directed to determine the exact amount of enzyme required to accomplish oxidation-reduction reaction so that the concentration of ethanol in the sample can be determined precisely and accurately. Results of the present study indicate that the lowest unit activity of the enzyme that can be used for ethanol determination isdoi:10.5267/j.ccl.2015.1.001 fatcat:oktem4ep5zfqhgynehhhwt3xse