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This study is designed to investigate the effect of temperature on thyroid gland of 24 adult healthy female carp fish (12/ season). Neutral buffered formalin solution10% was used for fixation and routine histological techniques. Hemotoxline and Eosine and PAS stains were, also used. The results revealed that the thyroid tissue in carp fish was closely associated with the renal and sub pharyngeal tissue. Spleen and liver did not show any thyroid follicles. The thyroid tissue of carp fishdoi:10.30539/iraqijvm.v40i1.140 fatcat:2zkbs3wugffldj4ae4zuhrgr34