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In situ 10-cell RNA sequencing in tissue and tumor biopsy samples
Single-cell transcriptomic methods classify new and existing cell types very effectively, but alternative approaches are needed to quantify the individual regulatory states of cells in their native tissue context. We combined the tissue preservation and single-cell resolution of laser capture with an improved preamplification procedure enabling RNA sequencing of 10 microdissected cells. This in situ 10-cell RNA sequencing (10cRNA-seq) can exploit fluorescent reporters of cell type indoi:10.1101/444182 fatcat:ydkxypcwhnddve7zws3ue5nnve