Isolation And Identification of Pseudomonas aeruginosa from Some Clinical And Environmental Samples And Study It,s Activity for The Production of Pyocyanin And Protease

Nazar Al-mamari, Adeba Al-Numa,an
2019 Journal of education and science  
The study includes isolation and identification of Pseudomonas aeruginosa from different sources as (90) samples were collected during a period from November (2017) to February (2018), including (wounds, suckers, urine, drinking water), Twinty five isolates of Pseudomonas aeruginosa were identified depending on morphological and biochemical tests at a rate of (27.77%) from total samples including (13) isolates from suckers used for sucking solutions from respiratory tract, (7) isolates from
more » ... ) isolates from wounds, (3) isolates from urine and (2) isolates from drinking water. The isolates from sucker formed the highest rate reached (14.44%) of total samples and (52%) of total Pseudomonas aeruginosa isolates, whereas the lowest rate was from drinking water (2.22%) of total samples and (8%) of total Pseudomonas aeruginosa isolates. The results showed that (92%) of total isolates were pyocyanin producer on King A agar medium, It was also found that all isolates of this bacteria have the ability to produce protease.
doi:10.33899/edusj.1970.163328 fatcat:umtalt376fg4pcjald47b32mqq