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The analysis of aberrant DNA methylation is used for the diagnosis of cancer as significant changes in the gene methylation pattern are often detected during early carcinogenesis. In this study, we evaluated the performance of a twostep method that combines pre-amplification with ddPCR technique. Results: By using ddPCR, the dependence of amplification efficiency for methylated and unmethylated DNA fragments on the relevant MgCl 2 concentration and the annealing temperature was established indoi:10.18632/oncotarget.26315 fatcat:32y7ojn6wbew5hiepyxu3yiima