Callus growth and essential oil of nilam (Pogostemon cablin (Blanco) Bth.) on the treatment with -naphtalen acetic acid and kinetin

GUNTUR TRIMULYONO, SOLICHATUN SOLICHATUN, SOERYA DEWI MARLIANA
2004 Biofarmasi Journal of Natural Product Biochemistry  
The objectives of the research were to study the effect of adding of α - naphtalen acetic acid (NAA) and kinetin on callus growth and essensial oil production from nilam (Pogostemon cablin (Blanco) Bth.) callus culture. The outline of the research was the callus growth and secondary metabolite production from plant's body could be triggered by the occurrence of plant growth regulation. The addition NAA and kinetin in culture's medium would influence cell proliferation and synthesis of protein,
more » ... o that both can induce callus growth and secondary metabolism production from the cell that have been cultured. According to the research objectives, the research was done by using in vitro callus culture method to obtain callus from explant P. cablin leaf and to induce essensial oil production. In vitro culture process consist of two stages. First stage was the callus initiation medium induced the callus from explant. The experiment was done by medium Murashige-Skoog (MS) with 2,4-D 0,5 mg/l and kinetin 0.5 mg/l; and the second stage was the medium treatment induced callus growth and essensial oil production. The research used factorial completely randomized design with two factors (NAA concentration: 0mg/l, 0,5 mg/l, 1,0 mg/l, 2,0 mg/l; and kinetin concentration: 0 mg/l, 0,5 mg/l, 1,0 mg/l, 2,0 mg/l) with 3 replicates. The collected data were qualitative data (callus morphology include texture and colour callus) and quantitative data (callus growth rate, callus dry weight and essensial oil content). Data were analyzed by Anova and followed by DMRT 5% confidence level and correlation regretion. The result of the research indicated the treatment with addition plant growth regulation (NAA and kinetin) on Murashige-Skoog's medium had significant effect on callus growth rate but it didn't have significant effect on callus dry weight and the increase of produced essential oil.
doi:10.13057/biofar/f020102 fatcat:fganyhbsvjfczfglzyryunn5la