Effect of Chelation with Calcium Disodium EDTA on Haemato-biochemical and Trace Mineral Profile in Blood from Lead Exposed Calves

R. C. Patra, D. Swarup
2005 Asian-Australasian Journal of Animal Sciences  
An experiment was performed using 20 calves of about one-month old to investigate the effect of chelation therapy with calcium disodium ethylenediaminetetraacetate (CaNa 2 EDTA) alone or along with antioxidant α-tocopherol in lead loaded calves on blood trace minerals, erythrocytic sulfahydryl groups and some haematobiochemical parameters. Fifteen calves were given lead orally at a daily dose of 7.5 mg of 99% pure lead acetate/kg body weight for 28 days. Then the lead was withdrawn on day 28
more » ... hdrawn on day 28 and the calves were randomly divided into three groups. Each group of five animals was either treated with CaNa 2 EDTA alone at the dose rate of 110 mg/kg body weight in two divided doses for 4 days or along with α-tocopherol at the dose rate of 100 mg/kg body weight orally daily for 7 days, keeping the remaining five calves as lead-exposed untreated controls. Blood samples were collected at the end of the lead exposure (day 0) and thereafter on day 2, 4, 7 and 10 from the start of the chelation treatment. The treatment with EDTA alone led to slow but non-significant improvement in blood copper level, but incorporation of antioxidant α-tocopherol in chelation therapy resulted in its significant decline, as recorded on day 7-post treatment. Withdrawal of lead or treatment with CaNa 2 EDTA alone or along with αtocopherol enhanced the erythrocytic thiol contents and the levels of T-SH and P-SH became statistically (p<0.05) comparable to those of lead-exposed controls by day 7 and 4, respectively. There was no significant (p>0.05) change in serum urea, creatinine, total protein and albumin levels between the treatment groups. It is concluded from the present investigation that treatment with CaNa 2 EDTA at the present dose rate is safe to be used for chelation in lead loaded calves. (Asian-Aust.
doi:10.5713/ajas.2005.1130 fatcat:w6pipsrpnzbqxewyfbxrrqtg2i