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An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning was used with a prism-pair compressor to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The delivery of broad-bandwidth ͑ϳ100 nm͒, sub-12-fs pulses was confirmed by interferometric autocorrelation measurements at the focal plane. A comparison of two-photon imaging withdoi:10.1117/1.3059629 pmid:19256690 fatcat:pwgh7jl6lvfqtow2a3vubltamu