Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

Inne Suherna Sasmita, Warta Dewi, Indrati dewi
2017 International Journal of Sciences  
Objective: to test the extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.) As dye substitution in Gram staining technique. Material and Methods: Made Preparations from the suspension Escherichia coli ATCC 25922 and Streptococcus sanguine ATCC 10556 counted 30 preparation, and then done Gram staining process to this preparation. 1 preparation using counterstain basic fuchsin as a control and 29 other preparations using ethyl acetate extract of Red Fruit as a counterstain, the result of
more » ... taining compared to using standard degradation of RHS color chart. Result: From 29 sample preparations used the counterstain extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.), 12 preparations produced a color close to color code 62B on the standard degradation of RHS color chart that is moderate purplish pink. Conclusions: From this preliminary study that examined the extract of ethyl acetate Red Fruit as a counterstain on Gram staining resulted in a color contrast between Gram + and Grambacteria though not as good as if using compressed/counterstain basic fuchsin.
doi:10.18483/ijsci.1357 fatcat:bkarmwhlifg7xfhzkma7j4fygi