Ocotea porosa: Anatomy and Histochemistry of Leaves and Stems, Chemical Composition, Cytotoxicity and Insecticidal Activities of Essential Oil

Libardone José Ribeiro Brustulim, Luciane Mendes Monteiro, Valter Paes de Almeida, Vijayasankar Raman, Beatriz Helena Lameiro de Noronha Sales Maia, Inaiara Casapula, Kátia Sabrina Paludo, Julia Emília Bussade, Junaid Ur Rehman, Ikhlas Ahmed Kahn, Paulo Vitor Farago, Jane Manfron Budel
2020 Brazilian Archives of Biology and Technology  
Ocotea porosa (Nees & Mart.) Barroso, commonly known as "imbuia", "canela-imbuia" or "imbuiaamarela" in Brazil, is a tree of the Southern Atlantic Forest. The present study investigates the anatomy of leaf and stem, volatile oil chemistry, as well as cytotoxicity and insecticidal activities of the essential oil of O. porosa. Species identification was achieved by anatomy features, mainly due to paracytic and anomocytic stomata; non-glandular trichomes; biconvex midrib and petiole with a
more » ... iole with a collateral open arc vascular bundle; presence of a sclerenchymatous layer, starch grains and crystal sand in the stem; and the presence of HIGHLIGHTS  The anatomy features were useful for identification of Ocotea porosa.  The major volatile compounds were α-pinene, β-pinene and bicyclogermacrene.  Essential oil of O. porosa was cytotoxic against McCoy, B16F10 and MCF7cell lines.  The cytotoxic mechanism might be related to apoptotic events.  Insert 1-4 highlights no longer than 85 characters.  Insert a highlight no longer than 85 characters.  Insert a highlight no longer than 85 characters. • Insert a highlight no longer than 85 characters. 2 Brustulim, L.J. R, et.al. phenolic compounds in the epidermis, phloem and xylem of the midrib, petiole and stem. The main volatile components of the essential oil were α-pinene (19.71%), β-pinene (13.86%) and bicyclogermacrene (24.62%). Cytotoxicity against human cancer cell (MCF-7), mouse cancer cell (B16F10) and mouse nontumoral cell (McCoy) was observed as well as insecticidal activity of the essential oil against susceptible 'Ft. Dix' bed bugs (Cimex lectularius L.) by topical application.
doi:10.1590/1678-4324-2020190082 fatcat:t5252ixwt5hzpku3ma46i56vhe