Evidentiation of Paramyosin (Sm-97) as a Modulating Antigen on Granulomatous Hypersensitivity to Schistosoma mansoni Eggs

Cristine Hirsch, Claudia Carvalho-Queiroz, Gloria R Franco, Sergio DJ Pena, Andrew JG Simpson, Alfredo M Goes
1997 Memórias do Instituto Oswaldo Cruz  
A Schistosoma mansoni adult worm anionic fraction (PIII) has previously been shown to protect mice against challenge infection and to reduce pulmonary and hepatic granulomatous hypersensitivity. Serum from PIII-immunized rabbit was used to screen a λgt11 cDNA library from S. mansoni adult worm in order to identify antigens capable of modulating granulomatous hypersensitivity. We obtained four clones with 400 (Sm-III.11), 900 (Sm-III.16), 1100 (Sm-III.10) and 1300 (Sm-III.12) bp of length. All
more » ... bp of length. All clone-specific antibodies were able to recognize most of the PIII components. The sequence analysis showed that these clones presented high homology with S. mansoni paramyosin . These findings ascribe a new function to this antigen with an important role in modulation of granulomatous hypersensitivity to S. mansoni eggs.
doi:10.1590/s0074-02761997000500020 pmid:9566237 fatcat:bkeyj54zv5ds5b3md5jjrqvdtq