Estimation of antioxidant properties of experimental poly-component complexes

N. Raksha, Ju. Sokolovskaya, E. Manzhaliy, D. Dobryanskiy, O. Savchuk
2020 Vestnik Kievskogo nacionalnogo universiteta imeni Tarasa Ševčenko  
The involvement of free radicals appears to be the feature of most human diseases. The general mechanism of cell damage involves the excessive uncontrolled production of reactive oxygen species resulting in the intensification of lipid peroxidation processes and damaging of macromolecules. These processes are generally accompanied by a decrease in the concentration and/or activity of natural antioxidants. Moreover, the exhaustion of the antioxidant capacity of the organism is among the key
more » ... among the key reasons leading to the development of pathological states. The maintenance of the prooxidant-antioxidant balance by the systematic use of products or dietary supplements, the components of which exhibit antioxidant activity, can be considered as a part of a strategy for the prevention and control of diseases associated with oxidative stress. The current work aims to study the free radical scavenging activity of two experimental poly-component complexes. Antioxidant properties of complexes were determined in experiments in vitro. The estimation of total antioxidant activity, nitric oxide, hydrogen peroxide radical, and superoxide anion scavenging activity was performed. The results revealed that both complexes have the potentials to prevent the formation of free radicals and can be used as agents with antioxidant properties. It was established that the effect of complex 1 and 2 is complex and first of all aimed at the prevention of the formation of dangerous free radicals by reducing the level of hydrogen peroxide. The additional mechanism involves the chelating of metal ions, in particular ferrous iron. The antioxidant ability exhibited by complex 1 was found to be higher than the total antioxidant activity of complex 2. Complex 1 was more effective in the ability to neutralize superoxide anion-radicals while complex 2 showed a high percentage inhibition of Fe2+-induced lipid peroxidation.
doi:10.17721/1728_2748.2020.82.63-66 fatcat:2iyoq2cwmvb3fl73xq7q6mnvda