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21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops Julius-Kühn-Archiv
In this study nested PCR assays have been developed for the detection of Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV) and Apple mosaic virus (ApMV) modifying a previously reported assay for the generic detection of ilarviruses. In all cases one generic upstream primer was used along with a virus-specific downstream primer in respective nested PCR assays. The application of the same thermocycling profile allowed all amplifications to run in parallel. Ilarvirus isolates fromfatcat:a7ilodicyndmnkzfoblra4uns4