Development of a Method for Detecting Coxiella burnetii in Cheese Samples

Akihiko HIRAI, Akiko NAKAMA, Takashi CHIBA, Akemi KAI
2012 Journal of Veterinary Medical Science  
Coxiella burnetii is the causative agent of Q fever, and the main route of infection in humans is inhalation of contaminated aerosols. Although oral transmission by contaminated raw milk or dairy products is also a possible route of human infection, there have been few studies investigating the presence of C. burnetii in dairy products. We developed a new method of extracting DNA from cheese and detecting C. burnetii DNA in cheese samples with a nested PCR assay. The limit of detection was 6.0
more » ... detection was 6.0  10 2 C. burnetii particles per gram. We subsequently used this method to examine the presence of C. burnetii in cheese at commercial markets in Tokyo from June 2005 to December 2008. Twenty-eight of 147 cheese samples were found to be positive for C. burnetii DNA. However, when we assessed the viability of C. burnetii by inoculating mice with DNA-positive samples, all of the samples were found to be negative. Thus, the viability of C. burnetii appears to have been lost in these cheese samples.
doi:10.1292/jvms.11-0023 pmid:21979453 fatcat:mww23tfqere53iiiw2pmevw6om