In Vivo Biological Effects of Marine Biosilica on a Tibial Bone Defect in Rats

Matheus de Almeida Cruz, Paulo Roberto Gabbai-Armelin, Alan de França Santana, João Paulo dos Santos Prado, Ingrid Regina Avanzi, Julia Risso Parisi, Márcio Reis Custódio, Renata Neves Granito, Ana Claudia Muniz Renno
2020 Brazilian Archives of Biology and Technology  
Research on biomaterials of natural origin has gained prominence in the literature. Above all, marine sponges, due to their architecture and structural components, present a promising potential for the engineering of bone tissue. In vitro studies demonstrate that a biosilica of marine sponges has osteogenic potential. However, in vivo works are needed to elucidate the interaction of biosilica (BS) and bone tissue. The objective of the study was to evaluate the morphological and chemical
more » ... nd chemical characteristics of BS compared to Bioglass (BG) by scanning electron microscopy (SEM) and X-ray dispersive energy (EDX) spectroscopy. In addition, to evaluate the biological effects of BS, through an experimental model of tibial bone defect using histopathological, histomorphometric, immunohistochemical (IHC) and mechanical tests. SEM and EDX demonstrated the successful extraction of BS. Histopathological analysis demonstrated that Control Group (GC) had greater formation of newly formed bone tissue compared to BG and BS, yet BG bone neoformation was greater than BS. However, BS showed material degradation and granulation tissue formation, with HIGHLIGHTS  BS was isolated from specimens of the marine sponge Tedania ignis collected in the north coast of São Paulo, Brazil.  BS derived from marine sponges has been used as a promising raw material for bone grafts substitutes and tissue engineering proposes.  The present work successfully obtained crystalline BS from marine sponges, able of properly interacting with bone tissue.  The results are very inspiring toward further in vivo investigations. 2 Cruz, M. A..; et al. absence of inflammatory process and formation of fibrotic capsule. The results of histomorphometry corroborate with those of histopathology, where it is worth emphasizing the positive influence of BS in osteoblastic activity. IHQ demonstrated positive VEGF and TGF-β immunoexpression for GC, BS and BG. In the mechanical test no significant differences were found. The present results demonstrate the potential of BS in bone repair, further studies are needed other forms of presentation of BS are needed.
doi:10.1590/1678-4324-2020190084 fatcat:lguz2te545f4vmflfazt4qvd4y