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The total flavonoids of Houttuynia cordata are determined by UV-Vis spectrophotometry. The extraction condition of flavonoids from Houttuynia cordata includes: the extraction temperature is 70˚C, solid-liquid ratio is 1:20, solvent is 80% ethanol, extraction time is 65 min. We studied the antioxidation of flavonoids in Houttuynia cordata, and results showed that flavonoids had strong reducing capacity, hydroxyl free radical scavenging activity and the superoxide anion radical scavengingdoi:10.4236/msce.2016.42014 fatcat:e3cddoo7zbaw3otubl6rxf6tkq