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The present research work was conducted to find out the most suitable inter and intra spacing for Greenhouse transplantation of in vitro grown potato plantlets in peat moss soil in order to get maximum seed potato yield. There were nine different combinations of inter and intra row spacing (30x15, 30x10, 10x15, 20x5, 30x5, 10x5, 20x15, 10x10, 20x10cm) in completely randomized design. Regarding vegetative growth, maximum plant height i.e 59.01 cm, 58.92 cm, 57.01 cm was observed at 30cmx5cm,doi:10.31015/jaefs.2020.3.5 fatcat:633vfuyh5jf5xmmzba72fjpwi4