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This study was conducted on shoot tip explants of date palm cv. Barhee to investigate effect of bacterial cell wall components on initial callus growth. Three concentrations of bacterial media ( 0.5 and 1.0 and 2.5) v/v were used for growing callus by tissue culture technique . MS media was served as a control treatment. Results showed that all bacterial media treatments increased callus fresh weight and nitrogen concentration of media significantly as compared to that of MS control media.doi:10.37077/25200860.2017.5 fatcat:wboro7spjjaypm24trdn4j23hu