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Molecular interactions of optical clearing agents were investigated using a combination of molecular dynamics ͑MD͒ simulations and optical spectroscopy. For a series of sugar alcohols with low to high optical clearing potential, Raman spectroscopy and integrating sphere measurements were used to quantitatively characterize tissue water loss and reduction in light scattering following agent exposures. The rate of tissue water loss was found to correlate with agent optical clearing potential, butdoi:10.1117/1.3484748 pmid:21054084 fatcat:64ysbnl52bajhi74y63wqy7tyu