Aseptic culture of cyclamen tuber tissue

Hiroaki OKUMOTO, Shigetoshi TAKABAYASHI
1969 Engei Gakkai zasshi  
Explants of cyclamen tubers were cultured asep-3. NAA, when used alone, stimulated bud formation at 0.1 mg/l, and root formation at 1 mg/l. This root inducing effect of 1 mg/l NAA was shifted to a bud inducing effect when adenine sulfate was supplied, in combination, at 20 and 100 mg/l. When used alone, 100 mg/l adenine sulfate inhibited organ formation completely. However, most of the organs formed under the influence of 0.01 mg/l NAA were roots when the latter was given in the medium even
more » ... adenine sulfate at 20 mg/l. 4. Among the temperatures used, diurnal alternation of 20°C day and 10°C night temperature proved best for organ formation. 5. The possibility of applying this technique to vegetative propagation was discussed from a horticultural view point.
doi:10.2503/jjshs.38.178 fatcat:imrudbfxxzfjdkwdgsr7ivqi6y