SCREENING APPROACHES FOR METHANE MITIGATING POTENTIAL OF TANNIN-CONTAINING PLANTS UNDER IN VITRO RUMEN ENVIRONMENT

A. Jayanegara, M. Ridla, N. Nahrowi
2012 Journal of the Indonesian Tropical Animal Agriculture  
ABSTRAK Tujuan penelitian ini adalah untuk melakukan pemilahan terhadap hijauan mengandung tanin yang memiliki sifat menurunkan emisi gas metana dengan menggunakan pendekatan univariabel, bivariabel dan multivariabel (principal component analysis, PCA). Sampel hijauan berasal dari beberapa negara, yakni Indonesia (n = 27 spesies), Mongolia (n = 14), Swiss (n = 16) dan Jerman (n = 3). Hijauan diinkubasi secara in vitro dengan cairan rumen-buffer pada suhu 39 o C selama 24 jam. Total produksi gas
more » ... Total produksi gas diamati sebagai indikator kualitas hijauan dan emisi gas metana diukur. Hasil menunjukkan bahwa, berdasarkan pendekatan bivariabel, hijauan yang menghasilkan emisi metana rendah umumnya juga memiliki kualitas yang kurang baik atau rendah total produksi gasnya, kecuali Rhus typhina yakni sebesar 43 ml metana/200 mg bahan kering. Loading plot dari PCA menunjukkan bahwa semua fraksi fenolik hijauan berlawanan arah dengan gas metana dan total produksi gas. Hijauan yang berlawanan arah dengan produksi metana adalah Bergenia crassifolia, Swietenia mahagoni, Clidemia hirta, Peltiphyllum peltatum, Acacia villosa dan R. typhina. Dapat disimpulkan bahwa, untuk hijauan mengandung tanin, pemilahan berdasarkan pendekatan univariabel, bivariabel dan multivariabel terkait emisi gas metana menunjukkan hasil yang serupa. ABSTRACT The aim of the present study was to conduct univariate, bivariate and multivariate (principal component analysis, PCA) approaches in the screening of tannin-containing plants from various collection sites for their CH 4 mitigating properties. Plant samples were obtained from various collection sites in different countries, i.e. Indonesia (n = 27 species), Mongolia (n = 14), Switzerland (n = 16) and Germany (n = 3). The plants were incubated in vitro with buffered-rumen fluid at 39 o C for 24 h. Total gas production was recorded as an indicator of feed quality and emission of CH 4 was measured. Results showed that, based on bivariate screening, generally, plants possessed low CH 4 production had low quality or low total gas production except Rhus typhina, i.e. 43 ml/200 mg DM. The loading plot of PCA showed that all phenolic fractions were in the opposite direction with CH 4 and total gas production. Plants clustered together in reverse direction to that of CH 4 were Bergenia crassifolia root and leaf, Swietenia mahagoni, Clidemia hirta, Peltiphyllum peltatum, Acacia villosa and R. typhina. It was conluded that, for tannin-containing plants, screenings based on univariate, bivariate and multivariate approaches in relation to ruminal CH 4 emission led to similar results
doi:10.14710/jitaa.37.4.278-285 fatcat:o52663rujfenrdtiibia3h2pdq