A Novel External Esophageal Perfusion Model for Reflux-Associated Respiratory Symptoms

Qinzi Li, Lingfei Kong, Shuna Zhang, Zhaoshuang Zhong, Xiaofeng Liu, Jinou Wang, Jian Kang
2010 Pathobiology (Basel)  
acid-perfused than PBS-perfused animals. Histological evidence suggested esophageal and pneumonic inflammations were prominent in acid-treated animals. Conclusion: Repetitive, acid-perfused, esophageal events copied the animal models of reflux esophagitis, and elicited inflammatory responses in the airways and lungs of guinea pigs. Abstract Background and Objective: Gastroesophageal reflux disease (GERD) has been linked to a number of extra-esophageal symptoms and disorders, primarily in the
more » ... piratory tract. Current animal models of reflux esophagitis are adapted to diseases of the digestive system, rather than to refluxassociated respiratory symptoms. The aim of this study was to evaluate a novel external esophageal perfusion model to induce esophageal, tracheal and pneumonic histological injury similar to that associated with GERD. Methods: Twenty guinea pigs were randomized to the acid-treated or PBStreated group. Esophageal catheters were used to perfuse the esophageal lumen of guinea pigs with hydrochloric acid containing 1 g/l pepsin or PBS for 14 days. The total cell number and cell differential counts in bronchoalveolar lavage fluid (BALF) were determined 24 h after the last perfusion. Histological changes in the esophageal, tracheal and pneumonic tissues were observed by hematoxylin-eosin staining. Results: The numbers of lymphocytes, eosinophils and total inflammatory cells in the BALF were significantly higher in
doi:10.1159/000292650 pmid:20516732 fatcat:5zha5jckhjh5talh5ogew7zyeq