Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate

Draginja Pericin, Senka Madjarev-Popovic, Zuzana Vastag
2008 Acta Periodica Technologica  
The partitioning of acid protease produced by submerged fermentation of Penicillium roqueforti in the aqueous two-phase system polyethylene glycol/sodium phosphate was studied. In the system with 15% polyethylene glycol 4000/25% NaH 2 PO 4 favourable partition coefficient and the top phase yield were obtained, 2.651 and 56.51%, respectively. The purification of acid protease from crude enzyme was optimized by optimizing separation factor. Completely randomized factorial design was applied and
more » ... n was applied and the system 15.54% PEG 4000/20% NaH 2 PO 4 appeared to be optimal. Statistically calculated separation factor of 3.553, was similar to the experimentally obtained value (3.084), which indicates the possible application of the factorial design onto ATPS partition and purifycation.
doi:10.2298/apt0839171p fatcat:lc3cr5mh2fam7iecqeti5xe2m4