Enzyme-linked Immunosorbent Assays of Canine Apolipoproteins B-100 and A-I

Kenjirou MIYOSHI, Eiji UCHIDA, Masayoshi NIIYAMA
2000 Journal of Veterinary Medical Science  
An enzyme-linked immunosorbent assay (ELISA) for canine blood apolipoprotein (apo) B-100 and A-I was developed. The working range for the assay was 1.8 to 28.7 ng/well for apoB-100 and it was 50 to 410 ng/well for apoA-I. The intra-and inter-assay coefficients of variation for the assay for apoB-100 were 5.4 and 6.9%, respectively, and for apoA-I they were 5.8 and 10.6%, respectively. The average concentrations of apoB-100 and A-I in 25 beagles (males, aged 3-4 years) were 0.084 +/-0.028 (mean
more » ... 084 +/-0.028 (mean +/-SD) mg/ ml and 6.29 +/-1.55 mg/ml, respectively. The ratios of canine (C) apoB-100 to CapoA-I were 1.41 +/-0.58%. The respective concentrations in one case of hyperlipidemia with systemic atherosclerosis were 0.454 mg/ml and 11.28 mg/ml (a ratio of 4.03%). These values were larger than those of the controls. These results suggest that the measurements of CapoB-100 and A-I concentrations by this newly developed ELISA are helpful for diagnosis of lipidosis. KEY WORDS: apolipoprotein A-I, apolipoprotein B-100, arteriosclerosis, canine, enzyme-linked immunosorbent assay.
doi:10.1292/jvms.62.1269 pmid:11193342 fatcat:i43lq2t7g5cedmrz5owgsicaou