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Prechondrogenic rat limb buds contain two different molecular species of heparan sulfate proteoglycan, a smaller one (HSPG I»-A) and a larger one (HSPG III-A). Although a part of HSPG I-A was solubilized with phosphate-buffered saline (PBS) from the limb bud, HSPG III-A and a large part of HSPG I-A were resistant to extraction with the same solution. To examine whether these PBS-insoluble proteoglycans exist in a different way in the limb bud, proteoglycans were extracted from the PBS-insolubledoi:10.2220/biomedres.12.89 fatcat:6gjrll5umjc6horfmvfy3s2s3i