Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell

Yongzheng Cong, Khatereh Motamedchaboki, Santosh Misal, Yiran Liang, Amanda J. Guise, Thy Truong, Romain Huguet, Edward D. Plowey, Ying Zhu, Daniel Lopez-Ferrer, Ryan T. Kelly
2021 Chemical Science  
We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome...
doi:10.1039/d0sc03636f fatcat:j5j5b3gidzhx3i3lo5s26frv44