Synthesis of metal oxide nanoparticles includes different chemical/physical method, but it requires a tedious procedure, expensive chemicals, additional time, high cost equipments and also challenges our environment. The present work focuses on preparing NPs eco-friendly with the help of leaf and flower extract of Leucas Aspera. The structural and morphological properties are examined by XRD, SEM and EDAX. The formation of highly crystalline and pure nickel oxide particles is examined from

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... powder diffraction. SEM image exhibits NiO NPs in spherical shape and coral reef structure for leaf and flower extract. The chemical composition of the prepared samples is confirmed from EDX analysis. The antibacterial activity is studied over gram negative and gram positive bacteria. The results depict that NiO NPs synthesized using leaf extract have a good impact on gram positive bacteria.