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Methylated fatty acid (FA) compounds in the heartwood and bark of some softwood species, specifically Pinus sylvestris, Abies alba, Picea abies, and Larix decidua, grown in the Czech Republic were evaluated. Strong H2SO4 was used for methylation of the lipids. The highest content of lipid was found in P. abies bark (40.132 mg/g o.d. sample), and the lowest content was in A. alba wood (11.027 mg/g o.d. sample). The highest concentration of FAs was observed in L. decidua bark. The highestdoi:10.15376/biores.10.4.7715-7724 fatcat:ka3u654cwbhwrbruzenirhofz4