Blood exosomal miRNA profiling reveals the complexity of hepatocellular carcinoma and identifies potential biomarkers as differential diagnosis
Hepatocellular carcinoma (HCC) is one of leading causes of cancer-related deaths worldwide, but there is a shortage of effective biomarkers for its diagnosis. We aimed to explore exosomal miRNAs as potential biomarkers for HCC diagnosis. After the eliminations, this study included 89 patients from component analysis (PCA) analysis suggested that daily alcohol consumption could alter the blood exosomal miRNA profiles of HBV positive non-HCC patients through miR-3168 and miR-223-3p. The miRNA
... 3-3p. The miRNA profiles also revealed the tumor stages of HCC patients. High expression of miR-455-5p and miR-30c-5p, which significantly correlated with better overall survival in tumor tissues, could also be detected in blood exosomes. Two pairs of miRNAs (miR-584-5p/miR-106-3p and miR-628-3p/miR-941) showed 94.1% sensitivity and 68.4% specificity to differentiate HCC patients from non-HCC patients. The specificity of the combination was substantially influenced by alcohol consumption habits. This study suggested that blood exosomal miRNAs can be used as new non-invasive diagnostic tools for HCC. However, their accuracy could be affected by tumor stage and alcohol consumption habits. The data used to support the findings of this study are available from the corresponding author upon 15 request. exosomal microRNAs as diagnostic and prognostic biomarkers for hepatocellular carcinoma. J Cell Biochem 2018. 5. Peck-Radosavljevic M: Drug therapy for advanced-stage liver cancer. Liver Cancer 2014, 3(2):125-131. 6. Tsuchiya N, Sawada Y, Endo I, Saito K, Uemura Y, Nakatsura T: Biomarkers for the early diagnosis of hepatocellular carcinoma. World J Gastroenterol 2015, 21(37):10573-10583. 7. Song P, Tang Q, Feng X, Tang W: Biomarkers: evaluation of clinical utility in surveillance and early diagnosis for hepatocellular carcinoma. Scand J Clin Lab Invest Suppl 2016, 245:S70-76. 8. Jeon Y, Jang ES, Choi YS, Kim JW, Jeong SH: Glypican-3 level assessed by the enzyme-linked immunosorbent assay is inferior to alpha-fetoprotein level for hepatocellular carcinoma diagnosis. Clin Mol Hepatol 2016, 22(3):359-365. 9. Banales JM, Inarrairaegui M, Arbelaiz A, Milkiewicz P, Muntane J, Munoz-Bellvis L, La et al: Plasma extracellular vesicle microRNAs for pulmonary ground-glass nodules. Journal of extracellular vesicles 2019, 8(1):1663666. 22. Manri C, Yokoi T, Nishida H: Size-Selective Harvesting of Extracellular Vesicles for Strategic Analyses Towards Tumor Diagnoses. Appl Biochem Biotechnol 2017, 182(2):609-623. 23. Love MI, Huber W, Anders S: Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 2014, 15(12):550.