Establishment of an Embryonic Tip Regeneration System of Soybean

LIU Si-yan, LI Guang-long, QU Jing, GUAN Shu-yan, YAO Dan, WANG Pi-wu, LIU Si-chen, JIA Yang, GUAN Peng-fei, WANG Long-qing
2016 Brazilian Archives of Biology and Technology  
In order to establish an optimal soybean embryonic tip regeneration system that can serve as soybean genetic transformation receptor, and be used for the study of genetic function verification, the influences of single factor on the adventitious bud of embryonic tip induction, elongation and rooting stage, are researched and compared.The single factors includes seeds soaking time, different kinds of hormones, different concentration of hormone and different concentration of sucrose. By one-way
more » ... ucrose. By one-way ANOVA and LSD ad hoc test , the results show that, for the embryonic tip adventitious bud induction stage, 12h is the optimal seeds soaking time, 2.0mg·L -1 is the optimal concentration of 6-Benzyl Aminopurine(6-BA), for the embryonic tip adventitious bud elongation stage, 0.2mg·L -1 indole-3-butyric acid (IBA) is optimal and 2.0mg·L -1 Gibberellic acid (GA 3 ) is optimal, and for the adventitious bud of embryonic tip rooting stage, 2.0mg·L -1 IBA is optimal,the average rooting rate is 93.34%. An Optimal embryonic tip regeneration system is established, and optimum mediums in different stages are found.
doi:10.1590/1678-4324-2016160548 fatcat:ljns5bfcqvfk5feksyqpdqc7pu