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Transgenes can affect transgenic mice via transgene expression or via the socalled positional effect. DNA sequences can be localized in chromosomes using recently established mouse genomic databases. In this study, we describe a chromosomal mapping method that uses the genomic walking technique to analyze genomic sequences that flank transgenes, in combination with mouse genome database searches. Genomic DNA was collected from two transgenic mouse lines harboring pCAGGS-based transgenes, anddoi:10.1538/expanim.53.103 pmid:15153672 fatcat:a3he6hrqbjfnzhrlh6hwogxs6i