Quantification of the Effect of Different Levels of IOP in the Astroglia of the Rat Retina Ipsilateral and Contralateral to Experimental Glaucoma
Investigative Ophthalmology and Visual Science
PURPOSE. To analyze the effects of different levels of intraocular pressure (IOP) in the macroglia in ocular hypertension (OHT) and contralateral eyes at 3 weeks after laser photocoagulation and compare these with effects in age-matched control rats. METHODS. Adult Sprague-Dawley rats were divided into an agematched control (naive) group and an OHT group. Retinas were processed as whole mounts and immunostained with GFAP for analysis of the retinal macroglia. RESULTS. The area of the retina
... a of the retina occupied by astrocytes (AROA) was quantified. GFAP immunostaining showed common features in ipsilateral and contralateral eyes. First, although the astrocyte network maintained a star-shaped morphology, these cells had fewer secondary processes and thinner cell bodies and primary processes than did naive cells. Second, Müller cells appeared as punctate GFAPϩ structures among astrocytes. Third, there was a significant reduction of the AROA in ipsilateral and contralateral eyes compared with naive eyes. Ipsilateral eyes had significantly less AROA than did contralateral eyes. The decrease was greater for OHT eyes with higher IOP levels. CONCLUSIONS. OHT induces changes in the macroglia of contralateral eyes; thus, these fellow eyes should not be used as control. In eyes with OHT, there is a close relationship between IOP values and decreased AROA. (Invest Ophthalmol Vis Sci.