Comparative Study of In situ Hybridization and Immunohistochemistry for the Detection of Porcine Circovirus 2 in Formalin-Fixed, Paraffin-Embedded Tissues

Duyeol KIM, Yooncheol HA, Yong-Hoon LEE, Sungwon CHAE, Kichan LEE, Kiwon HAN, Junghyun KIM, Ju-Ho LEE, Sung-Hoon KIM, Kyu-Kye HWANG, Chanhee CHAE
2009 Journal of Veterinary Medical Science  
In situ hybridization and immunohistochemistry with different types of antibody (monoclonal vs. polyclonal, natural vs. synthetic) was compared to detect porcine circovirus 2 (PCV2) in formalin-fixed, paraffin-embedded tissues from pigs with experimentally and naturally occurring postweaning multisystemic wasting syndrome. PCV2 DNA and antigen was detected in tissues from both experimentally and naturally infected pigs by in situ hybridization and immunohistochemistry, respectively. Statistical
more » ... tively. Statistical evaluation revealed that more PCV2 positive signals were significantly detected in both experimentally and naturally infected pigs by in situ hybridization compared with immunohistochemistry (P<0.05). The results of this study demonstrated that in situ hybridization proved more sensitive than immunohistochemistry for the detection of PCV2 in formalin-fixed, paraffin-embedded lymph node tissues. KEY WORDS: immunohistochemistry, in situ hybridization, porcine circovirus.
doi:10.1292/jvms.71.1001 pmid:19652494 fatcat:n7yyh6rmhngwlbz764lvstpgge