Quantificação de sulfadimetoxina em leite por cromatografia líquida de alta eficiência

Carlos Willian Feltrin, Ana Maria de Souza Mello, João Gabriel Rosa dos Santos, Marcelo Volpatto Marques, Nair Maria Seibel, Luiz Antonio Mazzini Fontoura
2007 Química Nova  
Recebido em 2/2//06; aceito em 25/4/06; publicado na web em 30/8/06 SULFADIMETHOXYNE QUANTIFICATION IN MILK BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. A method for HPLC determination of sulfadimethoxyne in milk is presented. The analyte isolation and concentration were performed by solidphase extraction through a C-8 cartridge, pre-conditioned with hexane, methanol and water and eluted with MeOH. The recovery determination was done with a spiked solution of 20, 50 or 100 μg L -1 . In this
more » ... -1 . In this concentration range, the recovery was 83.2% with a RDS of 15.4%. For quantification, a Zorbax Eclipse XDB-C8 (4.6 mm x 150 mm, 5 μm), a mobile phase of MeCN: 0.01 mol L -1 KH 2 PO 4 aq. (1:4) , and a variable wavelength detector (275 nm) were used.
doi:10.1590/s0100-40422007000100018 fatcat:ymq3aplh55dnzbiztwburkhtye