Production, Purification and Physicochemical Properties of an Exo‑Polygalacturonase from Aspergillus niger SW06

Yu-ping Ma, Hui Hao, Zhi-fei Chen, Zhi-wei Zhao, Shun-hui Chen, Si-wen Sun, Chun-ping Xu
2016 Journal of the Brazilian Chemical Society  
In this study, exo-polygalacturonase (exo-PG) production from Aspergillus niger SW06 was optimized by central composition design and high amount of 21.51 units mL -1 could be achieved in optimizing growth conditions. Both gel filtration and ion exchange chromatography revealed a single exo-PG activity peak, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the purified protein showed a single band with a molecular mass of 66.2 kDa. The purified enzyme
more » ... ied enzyme exhibited maximal activity in the presence of 1% citrus pectin at the temperature of 55 °C and pH of 5.0. The enzyme was stable within the pH range of 3.0-5.0 and below 60 °C. The Michaelis constant (K m ) and maximum velocity (V max ) of the enzyme was found to be 0.58 mg mL -1 and 20.66 μmol (mL min) -1 , respectively. The thermostable and acidic nature for the activity of this exo-PG make it possible to have wide range of industrial applications.
doi:10.21577/0103-5053.20160210 fatcat:re5jusotlnem3jmu3dyewt7tva