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Considering the need for the Brazilian government to develop tools for environmental monitoring for biodiversity conservancy purposes in the national protected areas system, this paper focuses on determining reference site metrics (or baselines) for adapting the Index of Biotic Integrity (IBI) based on the fish assemblages in the Pantanal National Park (PNP). The habitats in the PNP were grouped into four categories: main rivers, corixos (channels connecting the floodplain), permanent bays, anddoi:10.1590/1982-0224-20170041 fatcat:n2eojwqhcje3hk4sd4tlpgkj5m