The SPS and Digitonin Tests Applied to Porcine Mycoplasmas
Acta Veterinaria Scandinavica
Acta vet. scand. 1975, 16, 474-4 '76. Brief Communication THE SPS AND DIGITONIN TESTS APPLIED TO PORCINE MYCOPLASMAS Members of the family Mycoplasmataceae, of the order Mycoplasmatales, have been found to be selectively lysed by sodiumpolyanethol-sulfonate (SPS) and digitonin, whereas members of the family Acholeplasmataceae, of the same order, are resistant. A growth inhibition test based on this phenomenon has been developed for use on solid medium. The method is recommended (Freundt et al.
... ed (Freundt et al. 1973) for differentiation purposes in routine mycoplasma work. In the present study the applicability of the SPS and digitonin tests for differentiation of porcine mycoplasmas has been examined. The material examined consisted of Danish isolates, viz., 100 Mycoplasma hyorhinis (M. hyor.), 40 Mycoplasma suipneumoniae (M. suip.), 20 Mycoplasma hyosynoviae (M. hyosyn.), and 9 Mycoplasma f'Iocculare (M. floc.) . All these isolates had been cloned twice on solid medium and their identity proved by serological growth inhibition test (Friis 1971) . Acholeplasma granularum (A. gran.) was represented by 7 strains obtained from Belgium * and identified by serological growth inhibition. For comparason, some recognised type or reference strains were included. A medium developed for cultivation of M. suip. (Friis 1975 ) was used for all strains except those of M. hyosyn., which were grown on a modified Hayflick's medium (Hayflick 1965) enriched with arginine and mucin: Distilled water, 475 ml; Bacto PPLO broth w/o CV, 10.3 g; autoclavlng at 1 atm. for 2 min; yeast extract, 25.0 ml; 0.5 % phenol red sol., 1.8 ml: penicillin-G, 120 mg; 5.6 % thallium acetate sol., 1.2 ml; arginine-mucin sol., 7.8 ml (an autoclaved aqueous solution containing 8 % argininium monochloride and 0.8 % Baoto mucin bacteriological (Difco) ) , Serum (equal parts of horse and pig serum) was added • Kindly supplied by Dr. Dekeyser, Institut National de Recherches Vetertnaires, Brussels.