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An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739<sup>T</sup> were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6–14.4 × 10<sup>5</sup> cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmaticdoi:10.17221/206/2009-cjfs fatcat:dbytctnkhvhhpbkdmjtgcol4v4