Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii

I. Hochel, J. Škvor
2010 Czech Journal of Food Sciences  
An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739<sup>T</sup> were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6&ndash;14.4 &times; 10<sup>5</sup> cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic
more » ... ding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.
doi:10.17221/206/2009-cjfs fatcat:dbytctnkhvhhpbkdmjtgcol4v4