Study of Seed Germination and Enzyme Metabolism during Allelopathic Interaction of Trianthemaportulacastrum L and Fenugreek Seeds

Pooja Patil, Swarupa Agnihotri
2019 Current Agriculture Research Journal  
Absract In the fields, it is commonly observed that fenugreek plants are not growing to their maturity in the vicinity of Trianthema portlacastrum L. In this context, present study was conducted in laboratory to observe allelopathic interaction between Trianthema and fenugreek. Aqueous extracts of seed, stem and leaf of Trianthema were made. Treatment of these aqueous extracts was given to fenugreek seeds. Germination study was performed by using petriplates. All the experiments were performed
more » ... nts were performed in triplicates. Pre-treatment of aqueous extracts of seed, stem and leaf of Trianthema. had caused decline in germination percentage, fresh weight, radical and coleoptile length of fenugreek seeds when compared to untreated seeds. At the same time, mean germination time was found to be more in pre-treated seeds compared to control seeds. After 24 hrs of treatment, enzymatic study was performed. Enzymes from antioxidant metabolism such as peroxidase, ascorbate peroxidase, superoxide dismutase and activity of DPPH was reduced in treated seeds than control seeds. Activity of catalase was increased after treatment of leachates. All the data is graphically presented with the standard deviation. Exogenous treatment of aqueous extracts of Trianthema has caused variation in saponin content of fenugreek seeds. This observation was recorded by using finger prints of HPTLC. Finger prints showed that content of saponin was also declined in treated seeds as compared to control. All these observations indicate the strong negative influence of Trianthema on growth and metabolism of fenugreek.
doi:10.12944/carj.7.1.11 fatcat:jvbhqdklifaznp2uo4w5vurcg4